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Pre-conference Workshops & Courses

Dates: Jan 12 & 13, 2017

Venue: The African Institute for Capacity Development (AICAD)

Capacity: 80 Participants

Application deadline: Jan 31st, 2017

SUMMARY OF COURSES

  Course title Instructors
1 Flow Cytometry: Understanding & applications
(25 Participants)
Prof. J. Paul Robinson, PhD
Professor of Biomedical Engineering,
Purdue University, Indiana, USA
2 Point of care diagnostics for resource-limited settings
(25 Participants)
Prof. Aman Russom, PhD
Associate Professor at KTH Royal Institute of Technology, Stockholm, Sweden
Prof. Jacqueline Linnes, PhD
Assistant Professor of Biomedical Engineering, Purdue University, Indiana, USA
3 In situ Proximity Ligation Assay
(25 Participants)
Prof. Masood Kamali-Moghaddam &
Prof. Ola Söderberg
Associate Professors, Department of Immunology, Genetics and Pathology (IGP)
Uppsala University
4 New insights into HIV infection
(25 Participants)
Prof. Cristina Mussini, MD
Director of the Infectious Diseases Clinics
University of Modena and Reggio Emilia School of Medicine

Course 1

Title: Flow Cytometry: Understanding & applications

Instructor:

Prof. J. Paul Robinson, Purdue University, West Lafayette, Indiana, USA

Summary

We propose a two day short course to serve as an introduction (first half) and advanced (second half) course to introduce the fundamentals and applications of flow cytometry across a range of life science applications. Flow cytometry has become a go-to technology for single cell analysis. The first half day will be for those who want to learn about the fundamentals of how flow cytometry works. It will assume no background in the field and bring you up to a firm level of knowledge. The afternoon course will introduce the instrumentation and demonstrate actual sample running and basic analysis. Day two will introduced more advanced areas including multicolor analysis, high throughput screening, advanced multiparameter analysis, spectral technologies, cell sorting (theory only) and clinical applications for example. This course will provide the participants with a strong background level of understanding of the technology, current limitations, and the future opportunities.

Introduction

This course provides an introduction to the fundamentals and applications of flow cytometry (FC) across a range of life-science applications. FC has become a go-to technology for single cell analysis. Those who want to learn about the fundamentals of how FC works, and the background to the field & applications will find this course attractive. This course will provide a strong background understanding of the technology, current limitations, and future opportunities.

Who should attend?

Our target audience are individuals who would like to expand their knowledge of flow cytometry as well individuals who would like to become more expert in the advanced areas of the technology.  This is an opportunity to learn about future applications as well as what the impact the technology may have on informatics, automation and clinical diagnostics, and the role quality control and differences across all current commercial technologies.

Benefits.

You will become a “mini-expert” in your institution by the end of this course. You will understand the unique terminology as well as all of the fundamental concepts that will be taught by experienced teachers who know how to impart knowledge in easy-to-understand ways. By the end of this course, participants will be confident that they will be well informed on this technology and have a strong perspective of how it fits or competes with other measurement technologies.

Contents.

First day
  1. Background to the invention of flow cytometry – We will review why it was invented and what problems it tried to solve. We will show a historical development of the field with an emphasis on fundamentals.
  2. How does does flow cytometry work? How do we measure light scatter (and what does it mean), how is fluorescence measured, what are the core modes of operation. How do the fluidic systems operate, and what are the electronics and what are their limitations?
  3. Fundamentals of data analysis in flow cytometry discussing the use of forward and side scatter, 1-5 parameter flow cytometry and introduction to basic multiparameter analysis.
  4. Fundamentals of Calibration of flow cytometers
  5. Introduction to Ligand/receptor interactions using flow cytometry
Demonstrations: (Day 1) (assistance by company rep)
  1. Calibration of a flow cytometer using beads
  2. Running multicolor samples and analyzing them
  3. Demonstrating the fundamental toolsets of a flow cytometer
Second day
  1. Introduction to Cell Sorting –  through to advanced applications of cell sorting discussing opportunities for stem cell sorting, as well as therapeutic applications.
  2. Advanced concepts in flow cytometry: Spectral flow cytometry – we will identify and discuss at least two current approaches to spectral analysis
  3. Analysis of extracellular vesicles and other nano-particles. A detailed review of flow cytometry methods
  4. High Throughput flow cytometry: We will outline current advances in high throughput systems including hardware approaches, software and applications. These will be based on available systems and not custom technologies.
  5. Multiplexing using beads: demonstration of cytokine analysis.
Demonstrations:
  1. High color flow cytometry )7-9 color analysis)
  2. DNA analysis (if we have samples available)
  3. Basic blood analysis (routine phenotyping)

Presenter Biography

J. Paul Robinson is the SVM Professor of Cytomics and Professor of Biomedical Engineering at Purdue University. He has been involved in developing and using flow cytometers for over 35 years.  For the past 27 years he has been teaching graduate courses in the field. He has edited 9 book focused on flow cytometry technologies and he is the editor-in-chief of Current Protocols in Cytometry.

Course 2

Title: Point of care diagnostics for resource-limited settings

Instructor/s:

Prof. Aman Russom, KTH Royal Institute of Technology, Stockholm, Sweden
Prof. Linnes Jacqueline, Purdue University, West Lafayette, Indiana, USA

Summary

POC diagnostics are poised to reshape the delivery of healthcare system in both high-resource and resource limited settings. In this workshop, we will give an overview of this rapidly growing field with focus on the use of microfluidic technologies that integrate complex biochemical analyses into automated systems for resource-limited settings. The fist portion of the course will focus on fundamental microfluidics and the second will integrate this information into hands-on experiments fabricating and testing microfluidic devices as we investigate current products and future directions in point of care diagnostics.

Who should attend?

Our target audience is individuals who would like to acquire or expand their knowledge of the emerging field of point-of-care diagnostics. Primarily target are graduate students and postdocs and well as clinicians, scientists, and engineers interested in infectious disease detection with a focus on microfluidic and paper-based technologies.

Benefits to attendees

The aim of this mini-course is to give an introduction to POC diagnostics and will highlight basic concepts of microfluidics with emphasis on POC applications. This course will focus on point-of-care (POC) diagnostics for infectious disease detection in resource-limited settings, device fabrication, available options and future directions. After this course, you will be able to describe the concept of “Lab-on-a-chip” and participate in scientific discussions regarding the usefulness of micro-and nanotechnology for a given biological problem at point of care. You will gain hands on experience fabricating microfluidic devices and will be able to identify major targets, sample matrices and preparation requirements as well as methods of detection and signal amplification. You will gain hands on experience fabricating microfluidic and paperfluidic devices as well as working with current POC technologies. For newcomers to this field, this is a good opportunity to learn and identify possible new application areas for your own current and future research.

Contents

  • Intro to POC diagnostics in resource-limited settings
  • Basic background in microfluidics and diagnostic detection (e.g. laminar flow defines mixing, sample sources/matrices, sample preparation requirements, signal types (visual, fluorescent, electrochemical), increasing signal-to-noise ratio)
  • Hands on device fabrication (e.g. soft lithography, wax barriers in paper)
  • Hands on testing of current technologies (e.g. Lateral flow rapid diagnostics, Cephiad GeneExpert)
  • Discussion of future POC technology directions (e.g. omics, syndromic management, multiplex detection of numerous pathogens, field-based molecular diagnostics in paper)

Presenter Biography

Aman Russom is Associate Professor at KTH Royal Institute of Technology, Stockholm Sweden. His research focuses on microfluidics with emphasis on sample preparation for clinical and point of care diagnostics and has more than 15 years experience in the field of microfluidics. For the past 5 years he has been teaching graduate courses and workshops in the field.

Jacqueline Linnes is an Assistant Professor of Biomedical Engineering at Purdue University in West Lafayette, Indiana, USA. Her laboratory develops real-time detection technologies to prevent, diagnose, and better understand the pathogenesis of diseases. She focuses on non-invasive sample collection and rapid detection of pathogens and biomolecules to efficiently diagnose a variety of environmental, bacterial, and viral pathogens at the point of care.

Course 3

Course Title: In situ Proximity Ligation Assay

Instructor/s:

Prof. Masood Kamali-Moghaddam, Uppsala University
Prof. Ola Söderberg, Uppsala University
1-2 Graduates, students to be assigned, Uppsala University

Summary

Proximity ligation assay (PLA) is a versatile technology for protein analyses. The technology can be used to detect proteins with high sensitivity and specificity in body fluids such as blood plasma and cerebrospinal fluids. The in situ format of PLA allows detection and localization of proteins, their interactions and post-translational modifications in cells and tissues on a single molecule level.

In in situ PLA the target molecules are recognized by a pair of primary antibodies. By employing standardised secondary antibodies equipped with DNA oligonucleotide arms we can leave the primary antibodies unmodified. The DNA oligonucleotides will then be hybridizing to two subsequently added connector DNA oligonucleotides and template two ligation events. The newly formed circular DNA molecule will then serve as a template for rolling circle amplification to create rolling circle products (RCP), a thread of ssDNA that falls into a randomly coiled structure of 0.5-1µM in diameter. The RCPs are then allowed to be hybridized to fluorescently tagged oligonucleotides and are visualized using a fluorescence microscope.

Who should attend?

Undergraduate and master students, graduate students and post-doctoral fellows/researcher who are currently involved in or plan to use image analyses in their research.

Experience in working with fixated cells and immunohistochemistry will be a plus. 

Benefits to attendees

Learn hands-on state-of-the-art and advanced technology for image analysis. Have possibility to attend lectures and seminars discussing related technologies for protein analyses.

Contents

First day
  • Lecture: Advanced Molecular Tools for Proteome Analyses
  • Introduction to in situ PLA (1)
  • Seminar/Discussion: Application of PLA-based Molecular Tools in research (required active participations of attendees)
  • Preparation of fixated cells
  • Permeabilization of cells
  • Blocking
  • Incubation with primary antibodies
Second Day
  • Introduction on in situ PLA (2)
  • Lecture on image analysis
  • Seminar/Discussion: Application of PLA-based Molecular Tools in research (required active participations of attendees)
  • Incubation with secondary antibodies
  • Ligation and rolling circle amplification
  • Microscopy imaging
  • Image analyses

Presenter Biography

Prof. Masood Kamali Moghaddam is a pharmacist and molecular biologist with expertise in PLA and is the inventor of a variant of the PLA technology. He is also the head PLA proteomics facility providing services on advanced molecular tools such as In situ and solid-phase PLA.

Prof. Ola Söderberg is a tumour biologist with long experience in molecular cancer research and a developer of molecular tools including the in situ PLA.

Course 4

Course Title: New insights into HIV infection

Instructors:

Organized by Prof. Cristina Mussini
Director of the Infectious Diseases Clinics
University of Modena and Reggio Emilia School of Medicine

Summary

The access to antiretroviral therapy has made HIV infection a chronic disease and this is true in high income countries, but it will be come true also in low and middle income countries. The extension of life expectancy will lead patients to develop non-AIDS serious events as cardiovascular diseases and cancer. Aim of the present course is to focus on what is new in term of knowledge, remaining gaps and future perspectives. Points that will be discussed are:

  1. Immunopathogenesis
  2. Diagnostic tools and access to testing
  3. Late presentation
  4. When to start treatment
  5. Serious Non AIDS events with particular attention to cardiovascular risk, bone and kidney diseases and cancer.
  6. Retention in care
  7. Mother to child transmission
  8. Possibility of eradication

Who should attend?

Clinicians involved in diagnosis and care of persons with HIV infection as nurses. Since in the course there will be a part on basic science, also researchers interested in a better understanding of HIV infection could attend.

Benefits to attendees

To be updated on the last basic and clinical research on HIV infection, and to share clinical approach among clinicians and researchers from different countries.

Contents

First day (4 lectures)
  1. Immunopathogenesis and role of chronic immune activation
  2. Diagnostic tools and access to testing
  3. Late presentation
  4. When to start treatment
Second Day (4 lectures)
  1. Serious Non AIDS events with particular attention to cardiovascular risk, bone and kidney diseases and cancer.
  2. Retention in care
  3. Mother to child transmission
  4. Possibility of eradication

Presenter Biography

Cristina Mussini, MD, is Full Professor in Infectious Diseases, Director of the I.D. Clinics and Director of the School of Specialization in I.D. at the University of Modena and Reggio Emilia (Italy). She has almost 30 years of experience in the treatment of patients with HIV, and is member of the Governing Board of the European AIDS Clinical Society, member of the panel of the EACS guidelines for antiretroviral therapy, and of the scientific committees of several international conferences. He was leading rapporteur for the clinical section at the Sydney world AIDS conference.